Quality Control PCR: A Method for Detecting Inhibitors of TaqDNA Polymerase

Quality control PCR: a method for detecting inhibitors of Taq DNA polymerase.

Design and Evaluation of a PCR Method for Detecting White Spot Syndrome Virus in Shrimp

Background: White spot syndrome virus (wssv) is the causing agent for white spot disease in shrimp and many crustaceans. This disease is highly contagious and can cause death within 3–10 days under normal culture conditions. Therefore, early diagnosis of the virus is a necessity. Materials and Methods: Primers were designed for three regions of the virus genome and one region of the shrimp geno...

application of upfc based on svpwm for power quality improvement

در سالهای اخیر،اختلالات کیفیت توان مهمترین موضوع می باشد که محققان زیادی را برای پیدا کردن راه حلی برای حل آن علاقه مند ساخته است.امروزه کیفیت توان در سیستم قدرت برای مراکز صنعتی،تجاری وکاربردهای بیمارستانی مسئله مهمی می باشد.مشکل ولتاژمثل شرایط افت ولتاژواضافه جریان ناشی از اتصال کوتاه مدار یا وقوع خطا در سیستم بیشتر مورد توجه می باشد. برای مطالعه افت ولتاژ واضافه جریان،محققان زیادی کار کرده ...

Post-PCR sterilization: a method to control carryover contamination for the polymerase chain reaction

We describe a photochemical procedure for the sterilization of polynucleotides that are created by the Polymerase Chain Reaction (PCR). The procedure is based upon the blockage of Taq DNA polymerase when it encounters a photochemically modified base in a polynucleotide strand. We have discovered reagents that can be added to a PCR reaction mixture prior to amplification and tolerate the thermal...

A rapid, simple method for detecting PCR failure.

Department of Medical Microbiology, St. Bartholomew's Hospital Medical College, London, EC1A 7BE, UK The polymerase chain reaction (PCR) holds great potential for the rapid and sensitive detection of pathogenic microorganisms. (1~ There has been much interest in detecting and preventing falsepositive PCR results owing to product carry-over(2); however, less attention has been paid to false-nega...